BioMemory Collection CNR-IBBR-SUB

General Info

Collection Code
CNR-IBBR-SUB
Collection Name
Rhizobacteria promoting plant growth and bacteria degrading toxic pollutants (SUB)
Institution Code
Collection Type
Cryopreserved collection
Owner Institution
Institute of Biosciences and Bioresources (IBBR) - Section of Naples, Department of AgriFood Sciences (DiSBA), National Research Council of Italy (CNR), Naples, IT
Bioresources
Bacteria
Main Taxa
Enterobacterales, Sphingobacteriales, Pseudomonadales, Flavobacteriales, Xanthomonadales, Actinomycetales.
Description
The collection contains endophytic bacteria isolated from rice plants grown in Italy and in the State of Mali (West Africa) and polycyclic aromatic hydrocarbon degrading bacteria isolated from the industrial zone of the port of Marghera (Venice).
Keywords
Microbiology; genomics; metabolomics.
Preservation Techniques
Microorganisms are cryopreserved at temperatures below freezing (-80 °C). To prevent damage to cells caused by ice crystal formation, cells are resuspended in growth media containing cryoprotectants such as glycerol prior to storage at -80 °C. Frozen microorganisms can be revived by thawing at 37 °C and inoculating into fresh culture medium.
Services
Preservation of bacterial cultures, management and quality assurance of microbial resources, bacterial taxonomy and characterization.
Competence / Expertise
Microbiology: growth and manipulation of bacteria. Molecular Biology: isolation and manipulation of nucleic acids; cloning and heterologous expression of genes of interest in model organisms (E. coli); gene expression analysis (RT-PCR, RNA-seq). Biochemistry: structural and functional analysis of proteins by SDS-PAGE, native-PAGE and western blot; UV-visible, fluorescent and circular dichroism spectroscopy; enzymatic assays (varius enzymes inclucing DNA polymerases); in vitro DNA-ligand interaction techniques by innovative techniques, as the Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI); fluorescence microscopy for in vivo study of bacteria, nematodes, and plants.
Quality
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Projects
MICRO4Legumes (“Il microbioma vegetale simbionte come strumento per il miglioramento delle leguminose foraggere”), D.M.n.89267 (Italian Ministry of Agriculture); COST Action CA18111 "Genome Editing in Plants - A Technology with Transformative Potential" (PlantEd); European Commission for funding the ABSTRESS project (FP7 KBBE-2011-289562).
Associations
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License
-
Rights Holder
CNR-IBBR
Access Rights
Not-for-profit use only.
Bibliographic Citation
Endophytes from African Rice (Oryza glaberrima L.) efficiently colonize Asian Rice (Oryza sativa L.) stimulating the activity of its antioxidant enzymes and increasing the content of nitrogen, carbon, and chlorophyll. Microorganisms 9, 1714 (2021). https://doi.org/10.3390/microorganisms9081714|The overproduction of indole-3-acetic acid (IAA) in endophytes up-regulates nitrogen-fixation in both bacterial cultures and inoculated rice plants. Microbial Ecology, 74, 441-452. https://doi: 10.1007/s00248-017-0948-4| http://www.cnr.it/ontology/cnr/individuo/prodotto/ID326828
URL
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People / Contributors

  National Research Council of Italy. All rights reserved.
  Credits