Collection Code

CNR-IBBR-SUB

Collection Name

Rhizobacteria promoting plant growth and bacteria degrading toxic pollutants (CNR-IBBR-SUB)

Institution Code

IBBR-NA-CNR  

Collection Permanent Identifier

bf6ef975-8cde-47ea-9037-9ca01d8d61ea  

Collection Type

Cryopreserved collection, Living organisms

Owner Institution

Institute of Biosciences and Bioresources (IBBR) - Section of Naples, Department of AgriFood Sciences (DiSBA), National Research Council of Italy (CNR), Naples, IT

Bioresources

Bacteria

Main Taxa

Enterobacterales, Sphingobacteriales, Pseudomonadales, Flavobacteriales, Xanthomonadales, Actinomycetales.

Description

The collection contains endophytic bacteria isolated from rice plants grown in Italy and in the State of Mali (West Africa) and polycyclic aromatic hydrocarbon degrading bacteria isolated from the industrial zone of the port of Marghera (Venice).

Keywords

Microbiology; genomics; metabolomics.

Preservation Techniques

Microorganisms are cryopreserved at temperatures below freezing (-80 °C). To prevent damage to cells caused by ice crystal formation, cells are resuspended in growth media containing cryoprotectants such as glycerol before storage at -80 °C. Frozen microorganisms can be revived by thawing at 37 °C and inoculating into a fresh culture medium.

Services

Preservation of bacterial cultures, management and quality assurance of microbial resources, bacterial taxonomy and characterization.

Competence / Expertise

Microbiology: growth and manipulation of bacteria. Molecular Biology: isolation and manipulation of nucleic acids; cloning and heterologous expression of genes of interest in model organisms (E. coli); gene expression analysis (RT-PCR, RNA-seq). Biochemistry: structural and functional analysis of proteins by SDS-PAGE, native-PAGE and western blot; UV-visible, fluorescent and circular dichroism spectroscopy; enzymatic assays (varius enzymes inclucing DNA polymerases); in vitro DNA-ligand interaction techniques by innovative techniques, as the Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI); fluorescence microscopy for in vivo study of bacteria, nematodes, and plants.

Quality

-

Projects

MICRO4Legumes (“Il microbioma vegetale simbionte come strumento per il miglioramento delle leguminose foraggere”), D.M.n.89267 (Italian Ministry of Agriculture); COST Action CA18111 "Genome Editing in Plants - A Technology with Transformative Potential" (PlantEd); European Commission for funding the ABSTRESS project (FP7 KBBE-2011-289562).

Associations

Endophytes from African Rice (Oryza glaberrima L.) efficiently colonize Asian Rice (Oryza sativa L.) stimulating the activity of its antioxidant enzymes and increasing the content of nitrogen, carbon, and chlorophyll. Microorganisms 9, 1714 (2021). https://doi.org/10.3390/microorganisms9081714|The overproduction of indole-3-acetic acid (IAA) in endophytes up-regulates nitrogen-fixation in both bacterial cultures and inoculated rice plants. Microbial Ecology, 74, 441-452. https://doi: 10.1007/s00248-017-0948-4| http://www.cnr.it/ontology/cnr/individuo/prodotto/ID326828

License

http://creativecommons.org/licenses/by-nc/4.0/legalcode  

Rights Holder

CNR-IBBR, Naples, Italy

Access Rights

For research use only.

Bibliographic Citation

Bianco C, Defez R (2023). Collection of hizobacteria promoting plant growth and bacteria degrading toxic pollutants (CNR-IBBR-SUB). Istituto di Bioscienze e Biorisorse, Consiglio Nazionale delle Ricerche, Napoli, Italy. GBIF occurrence dataset. [online] URL: https://scientific-collections.gbif.org/collection/bf6ef975-8cde-47ea-9037-9ca01d8d61ea

URL

https://scientific-collections.gbif.org/collection/bf6ef975-8cde-47ea-9037-9ca01d8d61ea