Collection Code
CNR-IBBR-CELITABASE
Collection Name
C.elegans Italian collection (CelITAbase)
Collection Permanent Identifier
-
Collection Type
Cryopreserved collection
Owner Institution
Institute of Biosciences and Bioresources (IBBR) - Section of Naples, Department of AgriFood Sciences (DiSBA), National Research Council of Italy (CNR), Naples, IT
Main Taxa
Caenorhabditis elegans
Description
The frozen collection consists in more than 3,000 transgenic and mutant strains of non-parasitic nematodes, Caenorhabditis elegans species, accumulated over forty years. The collection, established in early ’80s by Dr. Paolo Bazzicalupo, is kept as triplicate, is unique in Italy and supports numerous Italian groups. C. elegans is the only multicellular animal model that can be frozen and stabs are stable for decades.
Keywords
Genetics, animal model, invertebrate, non parasitic nematode.
Preservation Techniques
Cryopreservation of L1 larval stage worms in 1 mL freezing solution containing NaCl 0.1M, phosphate buffer pH6.2, Glycerol 30%.
Services
Distribution of strains to the Italian C. elegans community. Local conservation of strains from CAENORHABDITIS GENETICS CENTER (CGC). Genotyping and phenotyping.
Competence / Expertise
Maintenance of C. elegans strains, Genetic crosses, Molecular biology, Anatomical methods, Neurobiology, Toxicology, Implementation of nutraceuticals, Genetic screens, Drug screens, Transgenesis
Quality
Genotyping and phenotyping of strains before and after freezing, including viability test. Collection is maintained as triplicate copies, in 2 different -80° Ultrafreezer and 1 in L.N.
Projects
Multicentre Research Grant 656IT/cv2008.1224 from Compagnia di Sanpaolo, “Bando 2008 del Programma di Neuroscienze”; MERIT Grant RBNE08LN4P_002 from CNR/MIUR; Grant FaReBio di Qualità -Farmaci e reti Biotecnologiche di Qualità from Ministry of Economy; Grant BANDO RICERCA FINALIZZATA 2009 RF-2009-1475235 from Ministry of Health; Grant Misura 3.17-2008 from Regione Campania; Grant Exploratory Project GEP14141 from Italian Telethon Foundation; Grant BANDO RICERCA SCIENTIFICA E TECNOLOGICA 2015 2015.03003.021 from Fondazione Cassa di Risparmio di Perugia; Grant BeHEARD Technology Prizes Rare Disease Challenge; Grant Full Project GGP16203 from Italian Telethon Foundation; Grant H2020-FETOPEN-1-2016-2017 801338 from European Community; Grant Joint Bilateral Agreement CNR/RFBR from CNR; Grant SMA-Europe Operating Grant 2019 22757; Grant H2020-FETOPEN-PROACTIVE-EIC-05 NUMBER 952183 from European Community; Grant CHARGE Syndrome Foundation Research Grant Program 2021 from Charge Foundation; Research Grant AFM-TELETHON ANNUAL CALL FOR PROPOSALS 2022 from AFM-Telethon France.
Rights Holder
Elia Di Schiavi
Access Rights
Not-for-profit use only. All strains may not be redistributed outside of the recipient’s organization without our written consent. Some strains can be distributed by accepting to cite the paper in which it has been described. Some strains contain patented and/or exclusively licensed components and can be distributed only under a collaborative agreement.
